Western Blot: The HepG2 cell lysate (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PFKM antibody (1:500-1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PFKM / PFK-1 Antibody (clone AT2F11)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C175824-50
Catalog Number: LS-C175824
For bulk orders or custom formulations:
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- Description
- PFK-1 antibody LS-C175824 is an unconjugated mouse monoclonal antibody to human PFK-1 (PFKM). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 6-phosphofructo-1-kinase | GSD7 | PFK1 | PFKA | PFKX | Phosphofructokinase-M | PFKM | Phosphofructokinase 1 | PFK-1 | PFK-A | Phosphofructokinase, muscle | Phosphohexokinase
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P08237
- NCBI GenBank
- NM_000289 | NP_000280.1
- SwissProt
- P08237
Target
- Target
- Human PFKM / PFK-1
- Antigen Species
- Human
- Epitope
- Human PFKM
- Gene Name
- PFKM
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT2F11
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Recombinant human PFKM (1-780aa) purified from E. coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS