Left: Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (lane 1), brain stem (lane 2), cerebellum (lane 3), and spinal cord (lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by NF-LMCA, NF-MMCA and MCA-NF-HMCA, spectrin/fodrin by S/FMCA and Microtubule associated protein 2 by MAP2MCA. Right: western blot of similar preparations separated on 6% SDS-PAGE and processed for immunoblotting with Peripherin antibody at a dilution of 1:10,000. A clear band running at 57kDa apparent size is seen in the spinal cord and brain stem lanes, but not in the other regions, in which peripherin is a very minor component. The position of this band on the Coomassie stained preparation is a little higher on the 8% gel and is indicated by PeriMCA in the left panel.