LSBio
Pepsin Antibody (Lyophilized)

Western Blot of Goat anti-Pepsin Antibody. Lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Pepsin primary antibody at 1:1,000 overnight at 4 degrees C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Pepsin. Other band(s): None.

Pepsin Antibody (Lyophilized)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Pig
Format:Lyophilized
See all Pig Pepsin antibodies →
$579.00each
Catalog Number: LS-C209190-100

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Overview

Description
Pepsin antibody LS-C209190 is an unconjugated goat polyclonal antibody to pig Pepsin. Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Por
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:50:000 - 1:200000
Recommended Dilution: IHC
1:2000 - 1:10000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:5000 - 1:20000

Target

Target
Pig Pepsin
Antigen Species
Pig
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.