LSBio
Pepsin Antibody (Liquid)

Immunohistochemistry of goat anti-pepsin antibody Antigen Retrieval: HIER pH6.2 10X Primary Antibody: Goat Anti-Pepsin 1 hr 1:500 Secondary Antibody: Swine anti-goat HRP 45 min followed by Staining with DAB. Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).

Pepsin Antibody (Liquid)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Pig
Format:Liquid
See all Pig Pepsin antibodies →
$311.00each
Catalog Number: LS-C744795-25

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Overview

Description
Pepsin antibody LS-C744795 is an unconjugated goat polyclonal antibody to pig Pepsin. Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Por
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:50:000 - 1:200000
Recommended Dilution: IHC
1:2000 - 1:10000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:5000 - 1:20000

Target

Target
Pig Pepsin
Antigen Species
Pig
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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