Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
PECAM-1 / CD31 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Recombinant Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$323.00each
Catalog Number: LS-C675337-50
Catalog Number: LS-C675337
For bulk orders or custom formulations:
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- Description
- CD31 antibody LS-C675337 is an unconjugated rabbit recombinant monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM1 | PECAM-1 | GPIIA
- UniProt Number
- P16284
- NCBI GenBank
- NM_000442 | NP_000433.4
- SwissProt
- P16284
Target
- Target
- Human PECAM-1 / CD31
- Antigen Species
- Human
- Gene Name
- PECAM1
Antibody
- Host Species
- Rabbit
- Clonality
- Recombinant Monoclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthesized peptide
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- PBS|150 mM NaCl