Fluorescent confocal image of SY5Y cells stained with phospho-PDX1-T11 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-PDX1-T11 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-PDX1 immunosignal mainly to the nucleus.

PDX1 Antibody (phospho-Thr11)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Dot Blot, Immunofluorescence
Reactivity:	Human
Format:	Liquid

$401.00each

Catalog Number: LS-C163661-400

Unit Size

Quantity

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Overview

Description: PDX1 antibody LS-C163661 is an unconjugated rabbit polyclonal antibody to human PDX1 (pThr11). Validated for DB and IF.
Application: DB, IF
Reactivity: Hu
Predicted Reactivity: Mouse, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Glucose-sensitive factor | IPF1 | IPF-1 | IUF-1 | STF-1 | GSF | IDX-1 | Islet/duodenum homeobox-1 | IUF1 | MODY4 | PDX-1 | PDX1
Recommended Dilution: IF/ICC: 1:100
UniProt Number: P52945
NCBI GenBank: NM_000209 | NP_000200.1
SwissProt: P52945

Target

Target: Human PDX1
Antigen Species: Human
Epitope: This PDX1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T11 of human PDX1.
Gene Name: PDX1

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen Type: Peptide

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS