Western blot analysis of extracts of COLO320 cells, using P4HB antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PDI / P4HB Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C496690-20
Catalog Number: LS-C496690
For bulk orders or custom formulations:
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- Description
- P4HB antibody LS-C496690 is an unconjugated rabbit polyclonal antibody to human P4HB (PDI). Validated for Flow, IHC and WB.
- Application
- FC, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERBA2L | DSI | GIT | p4HB | p4Hbeta | PDIA1 | PHDB | PO4DB | PROHB | Protocollagen hydroxylase | Protein disulfide-isomerase | PO4HB | PDI
- Recommended Dilution: FC
- 1:20 - 1:50
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P07237
- NCBI GenBank
- NM_000918 | NP_000909.2
- SwissProt
- P07237
Target
- Target
- Human PDI / P4HB
- Antigen Species
- Human
- Gene Name
- P4HB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide of human P4HB
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C, avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS