P4HB was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-P4HB rabbit monoclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
PDI / P4HB Antibody (clone 16, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C819116-100
Catalog Number: LS-C819116
For bulk orders or custom formulations:
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- Description
- P4HB antibody LS-C819116 is an unconjugated rabbit monoclonal antibody to human P4HB (PDI). Validated for ELISA, ICC, IF, IP and WB.
- Application
- ELISA, ICC, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ERBA2L | DSI | GIT | p4HB | p4Hbeta | PDIA1 | PHDB | PO4DB | PROHB | Protocollagen hydroxylase | Protein disulfide-isomerase | PO4HB | PDI
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P07237
- NCBI GenBank
- NM_000918 | NP_000909.2
- SwissProt
- P07237
Target
- Target
- Human PDI / P4HB
- Antigen Species
- Human
- Epitope
- Human P4HB.
- Gene Name
- P4HB
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 16
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human P4HB protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS