PDGFRA / PDGFR Alpha Antibody (Extracellular Domain, clone 35248 (57/20))
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$286.00each
Catalog Number: LS-C11204-0.1
Catalog Number: LS-C11204
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- Description
- PDGFR Alpha antibody LS-C11204 is an unconjugated mouse monoclonal antibody to human PDGFR Alpha (PDGFRA) (Extracellular Domain). Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD140A | CD140a antigen | PDGFalphaR | PDGFR2 | PDGFRalpha | AlphaPDGFR | RHEPDGFRA | PDGF receptor alpha | PDGFR-2 | PDGFRA/BCR fusion | APDGFR | Pdgf alpha-receptor | PDGF-R-alpha | PDGFR-alpha | PDGFRA
- Recommended Dilution: ELISA
- 0.5 - 1 ug/ml
- Recommended Dilution: IHC-P
- 10 - 20 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- P16234
- NCBI GenBank
- NM_006206 | NP_006197.1
- SwissProt
- P16234
Target
- Target
- Human PDGFRA / PDGFR Alpha
- Antigen Species
- Human
- Epitope
- Recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kD single pass type I transmembrane glycoprotein. PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases. Has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 ug/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.
- Gene Name
- PDGFRA
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 35248 (57/20)
- Isotype
- IgG1
Immunogen
- Immunogen
- Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 5% Trehalose
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS