Western Blot of rabbit anti-PDCD antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: PDCD antibody at 1:1,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 52 kDa for PDCD.

PDCD4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$311.00each

Catalog Number: LS-C745303-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: PDCD4 antibody LS-C745303 is an unconjugated rabbit polyclonal antibody to PDCD4 from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Predicted Reactivity: Mouse, Rat, Xenopus
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: H731 | Nuclear antigen H731-like | Nuclear antigen H731 | PDCD4 | Protein 197/15a
Recommended Dilution: ELISA: 1:10000 - 1:120000
Recommended Dilution: IHC: 1:100 - 1:300
Recommended Dilution: WB: 1:1000 - 1:10000
UniProt Number: Q53EL6
NCBI GenBank: NM_014456 | NP_055271.2
SwissProt: Q53EL6

Target

Target: Human PDCD4
Antigen Species: Human
Epitope: A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
Gene Name: PDCD4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl