Cell lysates of 293T and U937 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PDCD1 / CD279 / PD-1 Antibody (aa21-167, clone 4F12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C93777-50
Catalog Number: LS-C93777
For bulk orders or custom formulations:
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- Description
- PD-1 antibody LS-C93777 is an unconjugated mouse monoclonal antibody to human PD-1 (PDCD1 / CD279) (aa21-167). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD279 | HPD-1 | HPD-l | PD1 | PDCD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q15116
- NCBI GenBank
- NM_005018 | NP_005009.2
- SwissProt
- Q15116
Target
- Target
- Human PDCD1 / CD279 / PD-1
- Antigen Species
- Human
- Gene Name
- PDCD1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 4F12
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human PDCD1 (21-167aa) purified from E. coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS