Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
PCDHG / Protocadherin Gamma Antibody (clone S159-5)
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- Protocadherin Gamma antibody LS-C775732 is an unconjugated mouse monoclonal antibody to Protocadherin Gamma (PCDHG) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Human PCDHG / Protocadherin Gamma
- Antigen Species
- Hu
- Epitope
- Detects ~100 kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- S159-5
- Isotype
- IgG1
Immunogen
- Immunogen
- Fusion protein corresponding to amino acids 808-931 (C-Terminus cytoplasmic constant domain) of mouse Protocadherin-gamma-A1
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS