LSBio
PathPlus™ SERPINF1 / PEDF Antibody (aa196-347)

Anti-PEDF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ SERPINF1 / PEDF Antibody (aa196-347)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2471-50

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Overview

Description
PEDF antibody LS-B2471 is an unconjugated mouse monoclonal antibody to human PEDF (SERPINF1) (aa196-347). Validated for ELISA, ICC, IHC and WB. Cited in 1 publication.
Application
ELISA, ICC, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
EPC-1 | OI12 | PEDF | SERPINF1 | Serpin F1 | PIG35 | OI6
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
P36955
SwissProt
P36955

Target

Target
SERPINF1 / PEDF
Antigen Species
Human
Specificity
Recognizes human GST-PEDF fusion protein with a MW of ~46kD.
Epitope
Recognizes human GST-PEDF fusion protein with a MW of ~46kD.
Gene Name
SERPINF1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Origin Species
Human

Immunogen

Immunogen
Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-Terminus was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.6, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Differentiation of human pluripotent stem cells to retinal pigmented epithelium in defined conditions using purified extracellular matrix proteins. Rowland TJ, Blaschke AJ, Buchholz DE, Hikita ST, Johnson LV, Clegg DO. Journal of tissue engineering and regenerative medicine. 2013 August;7:642-653. PubMed: 22514096

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