LSBio
PathPlus™ Polyclonal Rabbit anti-Human MCM2 Antibody (aa15-40, IHC, WB)

Anti-MCM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ Polyclonal Rabbit anti-Human MCM2 Antibody (aa15-40, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Format:Liquid
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$516.00each
Catalog Number: LS-B391-100

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Overview

Description
MCM2 antibody LS-B391 is an unconjugated rabbit polyclonal antibody to MCM2 (aa15-40) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Predicted Reactivity
Fungus / Yeast / Mold, Human, Mouse, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BM28 | Cell devision cycle-like 1 | D3S3194 | Cdc19 | Cyclin-like 1 | KIAA0030 | MCM2 | Nuclear protein BM28 | CDCL1 | MITOTIN
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P49736
SwissProt
P49736

Target

Target
MCM2
Antigen Species
Human
Specificity
Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27). A BLAST analysis was used to suggest cross reactivity with MCM2 proteins from human, mouse, rat and S. cerevisiae based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Epitope
Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27). A BLAST analysis was used to suggest cross reactivity with MCM2 proteins from human, mouse, rat and S. cerevisiae based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
MCM2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 15-40 of human MCM2 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.98 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nascimento EM, Cox CL, MacArthur S, Hussain S, Trotter M, Blanco S, Suraj M, Nichols J, Kbler B, Benitah SA, Hendrich B, Odom DT, Frye M. Nature cell biology. 2011;13:1395-405. PubMed: 22101514 PMC: PMC3242072

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