Monoclonal Mouse Biotin Antibody
Catalog Number: LS-C127042-200
200 µg / $755.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Format: Liquid
Anti-MUC16 / CA125 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PathPlus™ Monoclonal Mouse anti-Human MUC16 / CA125 Antibody (clone X325, Extracellular Domain, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$383.00each
Catalog Number: LS-B9362-0.1
Catalog Number: LS-B9362
For bulk orders or custom formulations:
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- Description
- CA125 antibody LS-B9362 is an unconjugated mouse monoclonal antibody to human CA125 (MUC16) (Extracellular Domain). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CA125 ovarian cancer antigen | CA125 | CA-125 | MUC-16 | MUC16 | Mucin-16
- Recommended Dilution: IHC-P
- 10 µg/ml
- UniProt Number
- Q8WXI7
- NCBI GenBank
- NM_024690 | NP_078966.2
- SwissProt
- Q8WXI7
Target
- Target
- MUC16 / CA125
- Antigen Species
- Human
- Specificity
- Recognizes the extracellular domain of ovarian cancer mucin CA125 (also known as MUC16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group B, similar to the M-11 antibody. May be used to detect CA125 in ELISA assays
- Epitope
- Recognizes the ovarian cancer mucin CA125 (also known as MUC16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group B, similar to the M-11 antibody. May be used to detect CA125 in ELISA assays.
- Gene Name
- MUC16
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- X325
- Origin Species
- Human
Immunogen
- Immunogen
- CA125 antigen purified from human ovarian carcinoma
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- TBS