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PathPlus™ Monoclonal Mouse anti-Human Basic Cytokeratin AE3 Antibody (clone AE-3, Concentrated, IHC, WB)

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ Monoclonal Mouse anti-Human Basic Cytokeratin AE3 Antibody (clone AE-3, Concentrated, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Basic Cytokeratin AE3 antibodies →
$383.00each
Catalog Number: LS-B5557-50

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Overview

Description
Basic Cytokeratin AE3 antibody LS-B5557 is an unconjugated mouse monoclonal antibody to human Basic Cytokeratin AE3. Validated for IHC and WB.
Application
IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1:100

Target

Target
Basic Cytokeratin AE3
Antigen Species
Human
Specificity
This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
Epitope
This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
AE-3

Immunogen

Immunogen
Human epidermal cells.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 2-8°C.
Recommended Storage Buffer
0.01m PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.