LSBio
PathPlus™ IL-10 Antibody (clone JES5-2A5)

Anti-IL-10 antibody IHC of mouse small intestine. Positive cytoplasmic staining within absorptive epithelium of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ IL-10 Antibody (clone JES5-2A5)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Goat, Mouse
Format:Liquid
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$505.00each
Catalog Number: LS-B4913-0.1

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Overview

Description
IL-10 antibody LS-B4913 is an unconjugated rat monoclonal antibody to IL-10 from mouse. It is reactive with mouse and goat. Validated for ELISA, Flow, IHC and WB. Cited in 1 publication.
Application
ELISA, FC, IHC, IHC-F, IHC-P, WB
Reactivity
Gt, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CSIF | GVHDS | IL-10 | IL10 | IL10A | Interleukin-10 | Interleukin 10 | TGIF
Recommended Dilution: ELISA
1:200 - 1:2000
Recommended Dilution: FC
1:10 - 1:100
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
P22301
NCBI GenBank
NM_000572 | NP_000563.1
SwissProt
P22301

Target

Target
IL-10
Antigen Species
Mouse
Specificity
Recognizes mouse Interleukin-10 (IL-10). IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.
Epitope
Recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells and a subset of stimulated T cells. IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response. Is reported to neutralize the activity of murine IL-10.
Gene Name
IL10

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
JES5-2A5
Origin Species
Human

Immunogen

Immunogen
Recombinant mouse IL-10.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Upon receipt, aliquot and store at -20°C. May be stored at 4°C for up to 1 month. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS
Skin-Derived Mesenchymal Stem Cells Alleviate Atherosclerosis via Modulating Macrophage Function. Li Q, Sun W, Wang X, Zhang K, Xi W, Gao P. Stem cells translational medicine. 2015 November;4:1294-301. PubMed: 26400926 PMC: PMC4622403
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