Western blot analysis of extracts of various cell lines, using PARK7 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PARK7 / DJ-1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C331187-20
Catalog Number: LS-C331187
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- Description
- DJ-1 antibody LS-C331187 is an unconjugated rabbit polyclonal antibody to DJ-1 (PARK7) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DJ-1 | DJ1 | Parkinson disease protein 7 | Park-7 | Oncogene DJ1 | PARK7 | Parkinson protein 7 | Protein DJ-1
- Recommended Dilution: IF/ICC
- 1:50 - 1:200|1:20 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:20 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q99497
- NCBI GenBank
- NM_007262 | NP_009193.2
- SwissProt
- Q99497
Target
- Target
- Human PARK7 / DJ-1
- Antigen Species
- Human
- Epitope
- Human PARK7 / DJ-1
- Gene Name
- PARK7
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1). MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS