Western blot of lysate from SH-SY5Y cell line, using Park2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
PARK2 / Parkin 2 Antibody (aa111-140)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C99184-400
Catalog Number: LS-C99184
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- Description
- Parkin 2 antibody LS-C99184 is an unconjugated rabbit polyclonal antibody to human Parkin 2 (PARK2) (aa111-140). Validated for Flow, IF, IHC and WB. Cited in 1 publication.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AR-JP | LPRS2 | PARK2 | Parkin | PDJ | Parkinson disease protein 2 | PRKN | PRKN2
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O60260
- NCBI GenBank
- NM_004562 | NP_004553.2
- SwissProt
- O60260
Target
- Target
- Human PARK2 / Parkin 2
- Antigen Species
- Human
- Epitope
- This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human Parkin.
- Gene Name
- PRKN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1.5 mg/mL
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Overexpression of parkin ameliorates dopaminergic neurodegeneration induced by 1- methyl-4-phenyl-1,2,3,6-tetrahydropyridine in mice. Bian M, Liu J, Hong X, Yu M, Huang Y, Sheng Z, Fei J, Huang F. PloS one. 2012;7:e39953. PubMed: 22792139 PMC: PMC3390003