Dot Blot of Rabbit anti-PAK 1/2/3 pT423 antibody. Antigen: phosphorylated and non-phosphorylated forms of the immunizing peptide. Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng as indicated. Primary antibody: PAK 1/2/3 pT423 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:40,000 for 45 min at RT.
PAK1 + PAK2 + PAK3 Antibody (phospho-Thr423)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18892-100
Catalog Number: LS-C18892
For bulk orders or custom formulations:
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- Description
- PAK1 + PAK2 + PAK3 antibody LS-C18892 is an unconjugated rabbit polyclonal antibody to human PAK1 + PAK2 + PAK3 (pThr423). Validated for DB, ELISA, IHC and WB.
- Application
- DB, ELISA, IB, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:4000 - 1:20000
- Recommended Dilution: WB
- 1:500 - 1:2000
Target
- Target
- Human PAK1 + PAK2 + PAK3
- Antigen Species
- Human
- Epitope
- This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA. Cross reactivity with PAK 1/2/3 from other species has not been determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -70°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl