Western blot analysis of extracts of various cell lines, using HCRTR1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.
OX1R / Orexin Receptor 1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C749722-20
Catalog Number: LS-C749722
For bulk orders or custom formulations:
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- Description
- Orexin Receptor 1 antibody LS-C749722 is an unconjugated rabbit polyclonal antibody to Orexin Receptor 1 (OX1R) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Hcrt receptor 1 | Hypocretin receptor 1 | Hypocretin type 1 receptor | Hypocretin receptor-1 | Orexin receptor type 1 | Orexin type 1 receptor | Orexin-1 receptor | OX1 receptor | Orexin a receptor | Orexin receptor-1 | OX-R1 | Orexin receptor 1 | Ox1-R | OX1R | HCRTR1 | Hypocretin (orexin) receptor 1 | Hypocretin receptor type 1 | Ox-1-R
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O43613
- NCBI GenBank
- NM_001525, NP_001516.2
- SwissProt
- O43613
Target
- Target
- Human OX1R / Orexin Receptor 1
- Antigen Species
- Hu
- Gene Name
- HCRTR1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2). HPLLFKSTARRARGSILGIWAVSLAIMVPQAAVMECSSVLPELANRTRLFSVCDERWADDLYPKIYHSCFFIVTYLAPLGLMAMAYFQIFRKLWGRQIPGT
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS