Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
OGT / O-GLCNAC Antibody (clone 9H6, RPE)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$543.00each
Catalog Number: LS-C775689-100
Catalog Number: LS-C775689
For bulk orders or custom formulations:
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- Description
- O-GLCNAC antibody LS-C775689 is an RPE-conjugated mouse monoclonal antibody to all species O-GLCNAC (OGT). Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Pan
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- HRNT1 | O-GLCNAC | OGT
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: IHC
- 1:25
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O15294
- NCBI GenBank
- AAB63466.1 | U77413
- SwissProt
- O15294
Target
- Target
- All species OGT / O-GLCNAC
- Antigen Species
- Pan-species / Cross-reactive
- Epitope
- Specific for B-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
- Gene Name
- OGT
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 9H6
- Isotype
- IgM
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synthetic Immunogen
Format
- Conjugate/Label
- Phycoerythrin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS