Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NPAS4 Monoclonal Antibody, Clone S408-79. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.
NXF / NPAS4 Antibody (clone S408-79, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$485.00each
Catalog Number: LS-C776241-100
Catalog Number: LS-C776241
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- Description
- NPAS4 antibody LS-C776241 is a biotin-conjugated mouse monoclonal antibody to NPAS4 (NXF) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BHLHe79 | Neuronal PAS domain protein 4 | Neuronal PAS4 | NPAS4 | Le-PAS | NXF | PASD10
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q8IUM7
- NCBI GenBank
- NP_849195.2
- SwissProt
- Q8IUM7
Target
- Target
- Human NXF / NPAS4
- Antigen Species
- Human
- Epitope
- Detects ~90 kDa.
- Gene Name
- NPAS4
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- S408-79
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Fusion protein amino acids 597-802 (C-Terminus) of rat Npas4.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS