HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.
NUCB1 / Nucleobindin Antibody (clone OTI1A5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C173489-100
Catalog Number: LS-C173489
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Nucleobindin antibody LS-C173489 is an unconjugated mouse monoclonal antibody to human Nucleobindin (NUCB1). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CALNUC | Nucleobindin-1 | NUCB1 | Nucleobindin 1 | NUC | Nucleobindin
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- Q02818
- NCBI GenBank
- NM_006184 | NP_006175.2
- SwissProt
- Q02818
Target
- Target
- Human NUCB1 / Nucleobindin
- Antigen Species
- Human
- Epitope
- Human NUCB1 / Nucleobindin
- Gene Name
- NUCB1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI1A5
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.93 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS