Immunoprecipitation(IP) of NRBP1 by using monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NRBP1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
NRBP1 / NRBP Antibody (clone OTI2A5, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C784272-100
Catalog Number: LS-C784272
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- Description
- NRBP antibody LS-C784272 is an unconjugated mouse monoclonal antibody to human NRBP (NRBP1). Validated for Flow, IHC, IP and WB.
- Application
- FC, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- MUDPNP | NRBP | NRBP1 | BCON3 | MADM
- UniProt Number
- Q9UHY1
- NCBI GenBank
- NM_013392 | NP_037524.1
- SwissProt
- Q9UHY1
Recommended Dilution
| Application | Dilution |
|---|---|
| FC | 1:100 |
| IHC | 1:50 |
| IP | 4 µg/ml |
| WB | 1:2000 |
Target
- Target
- Human NRBP1 / NRBP
- Antigen Species
- Human
- Gene Name
- NRBP1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI2A5
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS