Western blot analysis of extracts of various cell lines, using NR5A1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
NR5A1 / SF1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C408335-20
Catalog Number: LS-C408335
For bulk orders or custom formulations:
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- Description
- SF1 antibody LS-C408335 is an unconjugated rabbit polyclonal antibody to SF1 (NR5A1) from human. It is reactive with mouse and rat. Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AD4BP | Adrenal 4 binding protein | Adrenal 4-binding protein | ELP | Steroidogenic factor-1 | SRXY3 | Steroidogenic factor 1 | NR5A1 | Nuclear receptor AdBP4 | Nuclear receptor elp | POF7 | SF-1 | SPGF8 | Steroid hormone receptor Ad4BP | STF-1 | FTZ1 | FTZF1 | Fushi tarazu factor homolog 1
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:20 - 1:50
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q13285
- NCBI GenBank
- NP_004950.2 | U76388
- SwissProt
- Q13285
Target
- Target
- Human NR5A1 / SF1
- Antigen Species
- Human
- Epitope
- Human NR5A1 / SF1.
- Gene Name
- NR5A1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NR5A1 (NP_004950.2). MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKL
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS