Anti-THRB1 Antibody - Western Blot. Western blot of affinity purified anti-THRB1 antibody shows detection of in vitro translated THRB1 (lane 2) and human thyroid stable cell line expressing THTRb1 (lane 3). No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA1 (lane 5). The band at ~62 kD, indicated by the arrowheads, corresponds to THRB. Personal communication, H. Ying, NCI, Bethesda, MD.
NR1A2 / THRB Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C60141-100
Catalog Number: LS-C60141
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- Description
- THRB antibody LS-C60141 is an unconjugated rabbit polyclonal antibody to THRB (NR1A2) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IP and WB.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-erba beta2 | C-ERBA-2 | ERBA-BETA | GRTH | Oncogene ERBA2 | THRB | THRB2 | TR-BETA | THRB1 | NR1A2 | Thyroid hormone receptor beta | Trbeta1 | Trbeta2 | C-erba beta1 | C-ERBA-BETA | ERBA2 | PRTH | THR1 | Thyroid hormone receptor, beta | Trbeta
- Recommended Dilution: ELISA
- 1:20000 - 1:200000
- Recommended Dilution: IF/ICC
- 1:1000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10828
- NCBI GenBank
- NM_000461 | NP_000452.2
- SwissProt
- P10828
Target
- Target
- Human NR1A2 / THRB
- Antigen Species
- Human
- Epitope
- This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.
- Gene Name
- THRB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This TR Beta1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.15 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl