Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Natriuretic Peptide Receptor C primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.