Western blot of Nucleophosmin in human spleen tissue lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse spleen using Polyclonal Antibody to Nucleophosmin at 5 and 2 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.
NPM1 / NPM / Nucleophosmin Antibody (aa21-38)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Dog, Hamster, Horse, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148793-100
Catalog Number: LS-C148793
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- Description
- Nucleophosmin antibody LS-C148793 is an unconjugated rabbit polyclonal antibody to Nucleophosmin (NPM1 / NPM) (aa21-38) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Can, Ham, Hr, Hu, Ms, NHP, Por, Rb, Rt
- Predicted Reactivity
- Bovine
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | NPM1 | Numatrin | B23 | Nucleophosmin
- Recommended Dilution: IHC-P
- 5 µg/ml
- UniProt Number
- P06748
- NCBI GenBank
- NM_002520 | NP_002511.1
- SwissProt
- P06748
Target
- Target
- Human NPM1 / NPM / Nucleophosmin
- Antigen Species
- Human
- Epitope
- Human NPM1.
- Gene Name
- NPM1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Amino acids 21-38 (CELKADKDYHFKVDNDEN) of human Nucleophosmin were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Panda, Rabbit, Horse, Pig, Opossum, Platypus (100%); Bovine (94%).
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.05% BSA
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
- Recommended Storage Buffer
- PBS