LSBio
NO Tryptophan (Nitrotryptophan) Antibody (clone 4F8)

Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.

NO Tryptophan (Nitrotryptophan) Antibody (clone 4F8)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species NO Tryptophan (Nitrotryptophan) antibodies →
$507.00each
Catalog Number: LS-C775536-100

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Overview

Description
NO Tryptophan (Nitrotryptophan) antibody LS-C775536 is an unconjugated mouse monoclonal antibody to all species NO Tryptophan (Nitrotryptophan). Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Pan
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: IF/ICC
1:50
Recommended Dilution: WB
1:1000

Target

Target
All species NO Tryptophan (Nitrotryptophan)
Antigen Species
Pan
Epitope
Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
4F8
Isotype
IgG2b

Immunogen

Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Synth

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.