TPH1 / Tryptophan Hydroxylase Antibody
Catalog Number: LS-C132713-100
100 µg / $576.00
Antibody: Mouse Monoclonal
Applications: Western Blot
Reactivity: Human, Rabbit, Rat
Format: Lyophilized
Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.
NO Tryptophan (Nitrotryptophan) Antibody (clone 2D12, Atto 488)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$545.00each
Catalog Number: LS-C821078-100
Catalog Number: LS-C821078
For bulk orders or custom formulations:
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- Description
- NO Tryptophan (Nitrotryptophan) antibody LS-C821078 is an Atto 488-conjugated mouse monoclonal antibody to all species NO Tryptophan (Nitrotryptophan). Validated for ELISA, Flow, IF and WB.
- Application
- ELISA, FC, IF, WB
- Reactivity
- Pan
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: FC
- 1:50
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species NO Tryptophan (Nitrotryptophan)
- Antigen Species
- Pan-species / Cross-reactive
- Epitope
- Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 2D12
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synthetic Immunogen
Format
- Conjugate/Label
- Atto 488
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS
