LSBio
NO Tryptophan (Nitrotryptophan) Antibody (clone 2D12, APC)

Flow Cytometry analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

NO Tryptophan (Nitrotryptophan) Antibody (clone 2D12, APC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species NO Tryptophan (Nitrotryptophan) antibodies →
$544.00each
Catalog Number: LS-C775528-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
NO Tryptophan (Nitrotryptophan) antibody LS-C775528 is an APC-conjugated mouse monoclonal antibody to all species NO Tryptophan (Nitrotryptophan). Validated for ELISA, Flow, IF and WB.
Application
ELISA, FC, IF, WB
Reactivity
Pan
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: FC
1:50
Recommended Dilution: IF/ICC
1:50
Recommended Dilution: WB
1:1000

Target

Target
All species NO Tryptophan (Nitrotryptophan)
Antigen Species
Pan
Epitope
Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
2D12
Isotype
IgG1

Immunogen

Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Synth

Format

Conjugate/Label
APC
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.