Immunofluorescence staining of NLGN3 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NLGN3 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm and cell membrane.
NLGN3 / Neuroligin 3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C816810-100
Catalog Number: LS-C816810
For bulk orders or custom formulations:
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- Description
- Neuroligin 3 antibody LS-C816810 is an unconjugated rabbit polyclonal antibody to human Neuroligin 3 (NLGN3). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ASPGX1 | AUTSX1 | Gliotactin homolog | KIAA1480 | NLGN3 | HNL3 | Neuroligin 3 | Neuroligin-3 | NL3
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- UniProt Number
- Q9NZ94
- NCBI GenBank
- NM_018977 | NP_061850.2
- SwissProt
- Q9NZ94
Target
- Target
- Human NLGN3 / Neuroligin 3
- Antigen Species
- Human
- Epitope
- Human Neuroligin 3 / NLGN3
- Gene Name
- NLGN3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human Neuroligin 3 / NLGN3 fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS