Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa. Other Band(s): ~60kDa (BNIP3 complex).
NIP3 / BNIP3 Antibody (Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$487.00each
Catalog Number: LS-C776859-100
Catalog Number: LS-C776859
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- Description
- BNIP3 antibody LS-C776859 is a biotin-conjugated rabbit polyclonal antibody to BNIP3 (NIP3) from human. It is reactive with human and mouse. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BNIP3 | NIP3
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q12983
- NCBI GenBank
- NM_004052 | NP_004043.3
- SwissProt
- Q12983
Target
- Target
- Human NIP3 / BNIP3
- Antigen Species
- Human
- Epitope
- Predicted molecular weight at ~21.5 kDa. Observed bands at ~20-30 kDa and 50-60 kDa due to the homodimer complex of BNIP3.
- Gene Name
- BNIP3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the C-Terminus of human BNIP3
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS