HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.
NHEJ1 / XLF Antibody (clone OTI1E2)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate, Rat |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C337190-100
Catalog Number: LS-C337190
For bulk orders or custom formulations:
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- Description
- XLF antibody LS-C337190 is an unconjugated mouse monoclonal antibody to XLF (NHEJ1) from human. It is reactive with human, rat, dog and other species. Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Can, Hu, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Cernunnos | NHEJ1 | Protein cernunnos | XRCC4-like factor | XLF
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9H9Q4
- NCBI GenBank
- NM_024782 | NP_079058.1
- SwissProt
- Q9H9Q4
Target
- Target
- Human NHEJ1 / XLF
- Antigen Species
- Human
- Epitope
- Human NHEJ1 / XLF.
- Gene Name
- NHEJ1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI1E2
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.92 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS