Immunofluorescence staining of NGFR in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-rat NGFR polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.
NGFR / CD271 / TNR16 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Rat |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815497-100
Catalog Number: LS-C815497
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- TNR16 antibody LS-C815497 is an unconjugated rabbit polyclonal antibody to rat TNR16 (NGFR / CD271). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Rt
- Predicted Reactivity
- Human, Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD271 | CD271 antigen | gp80-LNGFR | Nerve growth factor receptor | p75 ICD | p75(NTR) | p75NTR | TNFR superfamily, member 16 | NGF receptor | NGFR | TNFRSF16 | p75-NTR
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P08138
- NCBI GenBank
- NM_002507 | NP_002498.1
- SwissProt
- P08138
Target
- Target
- Rat NGFR / CD271 / TNR16
- Antigen Species
- Rat
- Epitope
- Rat NGFR
- Gene Name
- NGFR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Rat NGFR/P75
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS