Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
NFKBIE / IKB Epsilon Antibody (phospho-Ser22)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C199101-50
Catalog Number: LS-C199101
For bulk orders or custom formulations:
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- Description
- IKB Epsilon antibody LS-C199101 is an unconjugated rabbit polyclonal antibody to IKB Epsilon (NFKBIE) (pSer22) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- I-kappa-B-epsilon | IkappaBepsilon | IKB Epsilon | IkB-epsilon | NF-kappa-BIE | NFKBIE | IkB-E | NF-kappa-B inhibitor epsilon | IKBE
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- O00221
- NCBI GenBank
- NM_004556 | NP_004547.2
- SwissProt
- O00221
Target
- Target
- Human NFKBIE / IKB Epsilon
- Antigen Species
- Human
- Epitope
- IkappaB-epsilon (Phospho-Ser22) Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at serine22.
- Gene Name
- NFKBIE
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. (AA Range: 131-180)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS|150 mM NaCl