Western Blot of rabbit Anti-Nrf2 antibody. Lane 1: HeLa Nuclear Extract. Lane 2: Jurkat whole cell. Load: 10 µg per lane. Primary antibody: Nrf2 antibody at 1.0 ug/ml for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP secondary antibody at 1:70,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 63 kDa for Nrf2.
NFE2L2 / NRF2 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745402-100
Catalog Number: LS-C745402
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- Description
- NRF2 antibody LS-C745402 is an unconjugated rabbit polyclonal antibody to NRF2 (NFE2L2) (C-Terminus) from mouse. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bov, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NFE2L2 | NF-E2-related factor 2 | NFE2-related factor 2 | NRF2
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q16236
- NCBI GenBank
- NM_006164, NP_006155.2
- SwissProt
- Q16236
Target
- Target
- Mouse NFE2L2 / NRF2
- Antigen Species
- Ms
- Epitope
- A BLAST analysis was used to suggest cross reactivity with Nrf2 proteins from human, mouse, rat and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- NFE2L2
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl