Anti-Neu2 Antibody - Western Blot. Western blot of Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ug loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kD). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:1000. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
NEU2 / Sialidase 2 Antibody (aa100-125)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C60084-100
Catalog Number: LS-C60084
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- Description
- Sialidase 2 antibody LS-C60084 is an unconjugated rabbit polyclonal antibody to human Sialidase 2 (NEU2) (aa100-125). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- N-acetyl-alpha-neuraminidase 2 | Sialidase-2 | SIAL2 | Cytosolic sialidase | NEU2 | Neuraminidase 2
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9Y3R4
- NCBI GenBank
- NM_005383 | NP_005374.2
- SwissProt
- Q9Y3R4
Target
- Target
- Human NEU2 / Sialidase 2
- Antigen Species
- Human
- Epitope
- This antibody reacts with human Neu2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established. Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2.
- Gene Name
- NEU2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human Neu2.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.9 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl