Western blot analysis of Nestin expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). Nestin at 230 kD was detected using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
NES / Nestin Antibody (aa252-290)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Horse, Human, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C408085-100
Catalog Number: LS-C408085
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- Description
- Nestin antibody LS-C408085 is an unconjugated rabbit polyclonal antibody to Nestin (NES) (aa252-290) from human. It is reactive with human, rat and horse. Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hr, Hu, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NES | Nestin | Nbla00170
- UniProt Number
- P48681
- NCBI GenBank
- CAA46780.1, X65964
- SwissProt
- P48681
Target
- Target
- Human NES / Nestin
- Antigen Species
- Hu
- Epitope
- CNS stem cells.
- Gene Name
- NES
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-Terminus of human Nestin (252-290 aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- NaCl