Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
NEFM / NF-M Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Chicken Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204562-100
Catalog Number: LS-C204562
For bulk orders or custom formulations:
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- Description
- NF-M antibody LS-C204562 is an unconjugated chicken polyclonal antibody to human NF-M (NEFM). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 160 kDa neurofilament protein | NEF3 | NEFM | NF-M | NFM | Neurofilament 3
- Recommended Dilution: IF/ICC
- 1:2000|1.43055555555556
- Recommended Dilution: WB
- 1:10000
- UniProt Number
- P07197
- NCBI GenBank
- NM_005382 | NP_005373.2
- SwissProt
- P07197
Target
- Target
- Human NEFM / NF-M
- Antigen Species
- Human
- Gene Name
- NEFM
Antibody
- Host Species
- Chicken
- Clonality
- Polyclonal
- Isotype
- IgY
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 10 mM Sodium Azide
- Concentration
- 21.5 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS