CBB shows lane of Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at ~200kDa, NF-M at ~145kDa and NF-L at ~68kDa; in larger species such as pig or human, NF-H runs somewhat slower at ~210kDa or 220kDa. The Chk lane shows a blot probed with chicken anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, RPCA-NF-H.