Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, MCA-NAP4 (lane 1), NEFH / NF-H antibody (Lane 2) and MCA-9B12 (lane 3). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200kDa.
NEFH / NF-H Antibody (clone AH1)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Chicken, Mammal (Other) |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204550-100
Catalog Number: LS-C204550
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- Description
- NF-H antibody LS-C204550 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from bovine. It is reactive with chicken and mammal. Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Ck, Mml
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H | NEFH
- Recommended Dilution: IF/ICC
- 1:1000|0.736111111111111
- Recommended Dilution: WB
- 1:10000
- UniProt Number
- P12036
- NCBI GenBank
- NM_021076 | NP_066554.2
- SwissProt
- P12036
Target
- Target
- Bovine NEFH / NF-H
- Antigen Species
- Bovine
- Epitope
- Clone AH1 recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
- Gene Name
- NEFH
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AH1
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Native NF-H purified from bovine spinal cord.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Ascites fluid.
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.