Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-NEDD1 in Western blot of Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
NEDD1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C153764-100
Catalog Number: LS-C153764
For bulk orders or custom formulations:
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- Description
- NEDD1 antibody LS-C153764 is an unconjugated rabbit polyclonal antibody to human NEDD1. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bird / Avian (Other), Bovine, Chicken, Common Marmoset, Cynomolgus Monkey, Fish (Other), Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GCP-WD | NEDD-1 | NEDD1 | TUBGCP7 | Protein NEDD1
- Recommended Dilution: ELISA
- 1:100000
- Recommended Dilution: WB
- 1:10000
- UniProt Number
- Q8NHV4
- SwissProt
- Q8NHV4
Target
- Target
- Human NEDD1
- Antigen Species
- Human
- Epitope
- This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
- Gene Name
- NEDD1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 40 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
