The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: LaCap cell lysate Lane 4.: HeLa cell lysate
NCS1 / Neuronal Calcium Sensor Antibody (clone AT11B8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755515-50
Catalog Number: LS-C755515
For bulk orders or custom formulations:
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- Description
- Neuronal Calcium Sensor antibody LS-C755515 is an unconjugated mouse monoclonal antibody to human Neuronal Calcium Sensor (NCS1). Validated for ELISA, Flow and WB.
- Application
- ELISA, FC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Frequenin (Drosophila) homolog | FREQ | FLUP | Frequenin homolog | NCS1 | Neuronal calcium sensor 1 | Frequenin homolog (Drosophila) | Frequenin-like protein | NCS-1
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P62166
- NCBI GenBank
- NM_014286 | NP_055101.2
- SwissProt
- P62166
Target
- Target
- Human NCS1 / Neuronal Calcium Sensor
- Antigen Species
- Human
- Gene Name
- NCS1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT11B8
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human NCS1 (1-190aa) purified from E. coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Recommended Storage Buffer
- PBS