Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod
NCL / Nucleolin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C803015-100
Catalog Number: LS-C803015
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Nucleolin antibody LS-C803015 is an unconjugated rabbit polyclonal antibody to Nucleolin (NCL) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NCL | Nucleolin | C23 | Protein C23
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P19338
- NCBI GenBank
- NM_005381 | NP_005372.2
- SwissProt
- P19338
Target
- Target
- Human NCL / Nucleolin
- Antigen Species
- Human
- Epitope
- NUCL Antibody detects endogenous levels of NUCL.
- Gene Name
- NCL
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human NUCL.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS