Western blot analysis of extracts from HeLa cells, HepG2 cells and A549 cells treated with PMA (125ng/ml, 30mins), using p47 phox (Ab-370) antibody.
NCF1 / p47phox / p47 phox Antibody (p370)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C823220-100
Catalog Number: LS-C823220
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- Description
- P47 phox antibody LS-C823220 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) (p370) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NCF1A | Neutrophil cytosolic factor 1 | NCF-47K | Nox organizer 2 | NOXO2 | p47-phox | p47phox | SH3PXD1A | NADPH oxidase organizer 2 | NCF-1 | NCF1 | Neutrophil cytosol factor 1 | Nox-organizing protein 2
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P14598
- NCBI GenBank
- NM_000265 | NP_000256.4
- SwissProt
- P14598
Target
- Target
- Human NCF1 / p47phox / p47 phox
- Antigen Species
- Human
- Epitope
- p47 phox (Ab-370) antibody detects endogenous levels of total p47 phox protein.
- Gene Name
- NCF1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human p47 phox around the phosphorylation site of serine 370(R-P-SP-A-D).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl