Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker; Lane 2.Reference control; Lane 3-4.Positive control, Hela and MD231 whole cell lysate; Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
NAMPT / Visfatin Antibody (Preservative Free, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$350.00each
Catalog Number: LS-C343569-50
Catalog Number: LS-C343569
For bulk orders or custom formulations:
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- Description
- Visfatin antibody LS-C343569 is a biotin-conjugated rabbit polyclonal antibody to human Visfatin (NAMPT). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- PBEF | Pre-B cell-enhancing factor | VISFATIN | VF | NAmPRTase | NAMPT | PBEF1
- Recommended Dilution: ELISA
- 1:1000000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P43490
- NCBI GenBank
- NM_005746 | NP_005737.1
- SwissProt
- P43490
Target
- Target
- Human NAMPT / Visfatin
- Antigen Species
- Human
- Epitope
- The rabbit anti-human Visfatin antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and HeLa at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
- Gene Name
- NAMPT
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide derived from human Visfatin. Immunogen Sequence: CVTKSYSFDEIRKNAQLNIELEAAHH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from PBS, No preservatives added
- Preservative
- No
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Short term: Store at 4 °C for 1 month. Long term: Store at -20°C to -70°C for up to 1 year. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS