Western blot analysis of human and mouse cell lines using anti-human NLRP1/NALP1 antibody (AL176) .Method: Total protein extracts (20 ug) from various human (293-T, Jurkat, Raji, Ramos, BJAB, THP-1, U937, K562, Raw, HeLa) and mouse (EL-4, A20) cell lines were run on SDS-PAGE and NLRP1/NALP1 detected using anti-human NLRP1/NALP1 antibody (AL176) at 1:500 dilution. Anti-rabbit IgG coupled horse radish peroxidase was used at 1:5000 dilution for ECL detection.
NALP1 / NLRP1 Antibody (aa2-25)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$458.00each
Catalog Number: LS-C345053-100
Catalog Number: LS-C345053
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- Description
- NLRP1 antibody LS-C345053 is an unconjugated rabbit polyclonal antibody to human NLRP1 (NALP1) (aa2-25). Validated for WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CLR17.1 | CARD7 | DEFCAP | DEFCAP-L/S | NAC | SLEV1 | VAMAS1 | KIAA0926 | NALP1 | NLRP1 | PP1044
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9C000
- NCBI GenBank
- NM_014922 | NP_055737.1
- SwissProt
- Q9C000
Target
- Target
- Human NALP1 / NLRP1
- Antigen Species
- Human
- Epitope
- Recognizes human NLRP1/NALP1. Does not cross-react with mouse NLRP1/NALP1.
- Gene Name
- NLRP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic peptide corresponding to aa 2-25 (A2GGAWGRLACYLEFLKKEELKEFQ25) at the N-terminus of human NLRP1/NALP1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, 10% Glycerol
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS