Immunofluorescence staining of NAA10 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NAA10 polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
NAA10 / ARD1A Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815520-100
Catalog Number: LS-C815520
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- Description
- ARD1A antibody LS-C815520 is an unconjugated rabbit polyclonal antibody to human ARD1A (NAA10). Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARD1A | ARD1 | DXS707 | NAA10 | N-alpha-acetyltransferase 10 | NATD | NatA catalytic subunit | TE2
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- Recommended Dilution: IHC-P
- 1:100 - 1:400
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P41227
- NCBI GenBank
- NM_003491 | NP_003482.1
- SwissProt
- P41227
Target
- Target
- Human NAA10 / ARD1A
- Antigen Species
- Human
- Epitope
- Human NAA10/ARD1
- Gene Name
- NAA10
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human ARD1A/NAA10
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS